Epstein-Barr Virus, Epigenetics, and Cancer

Welcome to the TEMPERA LAB

We are located in the Fels Institute for Cancer Research and Molecular Biology at the Lewis Katz School of Medicine – Temple University. We pursue two lines of research: one focus is on how chromatin can regulate the complex and dynamic gene expression patterns adopted by the oncovirus Epstein-Barr Virus (EBV) during persistence infection; a second focus is on an important family of polymerases, the poly-ADP ribose polymerases (PARPs), which regulate functions like DNA repair, genomic stability, and apoptosis.

We utilize our expertise in chromatin immunoprecipitation, Chromatin Conformation Capture (3C) assay, and genome-wide data analysis to better understand the link between three-dimensional structure of chromosome, chromatin composition and gene expression during EBV latency and PARP activation. We aim in the long run to identify new cellular functions regulated by EBV and PARP1 that can be targeted by epigenetic drugs and to provide novel therapeutic approaches for treating EBV+ cancer. Learn more

The Latest

November 2018 The paper from our collaborator Dr. Maria D’Erme, at the Department of Biochemical Science of Rome University “La Sapienza”, is now available on the Biochemical Pharmacology journal

November 2018 Our work connecting PARP1 activity with LMP1-driven gene expression and alteration of cellular metabolism is now available on the PLOS Pathogens website.

October 2018 The work of our collaborator Dr. Maria D’Erme has been accepted for publication in Biochemical Pharmacology. We are glad we were part of this interesting research about Poly(ADP-ribosylated) proteins in β-amyloid peptide-stimulated microglial cells.

October 2018 Our paper describing the importance of PARP1 for LMP1-mediated gene expression has been accepted for publication at PLOS Pathogens. Congratulations to Michael for his work.