Epstein-Barr Virus, Epigenetics, and Cancer

Welcome to the TEMPERA LAB

We are located in the Fels Institute for Cancer Research and Molecular Biology at the Lewis Katz School of Medicine – Temple University. We pursue two lines of research: one focus is on how chromatin can regulate the complex and dynamic gene expression patterns adopted by the oncovirus Epstein-Barr Virus (EBV) during persistence infection; a second focus is on an important family of polymerases, the poly-ADP ribose polymerases (PARPs), which regulate functions like DNA repair, genomic stability, and apoptosis.

We utilize our expertise in chromatin immunoprecipitation, Chromatin Conformation Capture (3C) assay, and genome-wide data analysis to better understand the link between three-dimensional structure of chromosome, chromatin composition and gene expression during EBV latency and PARP activation. We aim in the long run to identify new cellular functions regulated by EBV and PARP1 that can be targeted by epigenetic drugs and to provide novel therapeutic approaches for treating EBV+ cancer. Learn more

The Latest

October 2019 We have a Postdoctoral position available for those who are interested in studying the role of both human and viral 3D chromatin structure during EBV latency. This position combines wet lab experiments with bioinformatic analysis of Hi-C dataset. The ideal candidate should be able to perform and analyze Hi-C experiments and to collaborate with other members of the lab on projects involving 3D structure analysis. If you are interested to apply or you want to know more about the project please send an email at tempera@temple.edu

July 2019 We will present our work at the IHW2019 in Knoxville. On Saturday July 20 at 9:00 Sarah will present her work about the 3D structure of EBV; on Sunday Lisa, Sarah and Italo will present their posters: 4.07; 4.31, and 2.33 respectivelly. On Monday, finally MIke will present his poster #2.14.

May 2019 Welcome Vered Schwell, M.D./Ph.D student, to our lab. Vered will work on LMP1/PARP1 signaling in Epstein-Barr Virus infected B cells.

March 2019 We submitted our abstracts about different ways epigenetic modifications regulate EBV latency. See you at the IHW2019 in Knoxville.